Investigation of enzyme activity during seed germination: Methodology.

Broad bean seeds showong cotyledons.

• The germination process was accomplished in the laboratory by soaking half-broad bean ( Vicia faba ) seeds in starch and milk agar present in two petri dishes.
• A control was used, to compare the findings. This was achieved by boiling the barley seeds before putting them into the petri dish soaked with water.
• Four barley seeds are split in half, seperating the cotyledons.
• Two of the split seeds are killed by boiling, for 5 minutes. These are used as the controls.
• All the seeds are sterilised for ten minutes, by soaking in disinfectant solution.
•  The petri dishes are labelled so as to avoid confusion eg 'Raw and Boiled seeds.'
• The seed halves are placed face down, in the appropriate petri dishes containing filter paper which have been saturated with either starch or milk agar.
• The plates are incubated at room temperature ( 18-20 degrees celcius) for 48hours.
• The results were analysed.

Petri dishes with filter paper.